Cation-dependent Reassociation of Subunits of N10-Formyltetrahydrofolate Synthetase from Clostridium acidi-urici and Clostridium cylindrosporum
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Folate Coenzymes of Clostridium acidi-urici
(Z-)5,10-Methenyltetrahydropteroyltriglutamic acid was isolated from Clostridium acidi-urici. The procedure yielded approximately 25 mg of this stable derivative of the natural folate coenzyme per 100 g of wet cells. (I)-Tetrahydropteroyltriglutamate was synthesized by enzymic arsenolysis of the product. Procedures for the synthesis of (2)-10-[14C]formyltetrahydropteroyltriglutamate, (Z)-10-for...
متن کاملAcetate formation in Clostridium acidi-urici: acetokinase.
Sagers, Richard D. (University of Illinois, Urbana), Moshe Benziman, and I. C. Gunsalus. Acetate formation in Clostridium acidiurici: Acetokinase. J. Bacteriol. 82:233-238. 1961.-To define the energy-yielding reaction(s) during fermentation of purines by Clostridium acidi-urici, an eightfold purified acetokinase was obtained by protamine and ammonium sulfate fractionation of crude extracts. Enz...
متن کاملL-serine specific dehydrase from Clostridium acidi-urici.
Formation of serine and pyruvate as intermediates in uric acid fermentation by Clostridium acidi-urici was suggested by Radin and Barker (1953), Sagers and Beck (1956), and Rabinowitz and B3arker (1956) to account for the labeling of acetate formed from C14-labele(d purines, and from the ability of cell suspensions and extracts to deaminate serine to pyruvate and ammonia. The present communicat...
متن کاملFerredoxin and formyltetrahydrofolate synthetase: comparative studies with Clostridium acidiurici, Clostridium cylindrosporum, and newly isolated anaerobic uric acid-fermenting strains.
Six strains of Clostridium acidiurici and three strains of C. cylindrosporum were isolated from soil samples by enrichment culture with uric acid as the source of carbon, nitrogen, and energy. The newly isolated strains were characterized by their spore morphology and the amounts of glycine and formate formed by the fermentation of uric acid. The strains were easily identified as belonging to o...
متن کاملPhysical properties of formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
The hydrodynamic parameters of the thermostable formyltetrahydrofolate synthetase from Clostridium thermoaceticum have been measured in the ultracentrifuge at 20”. &J,~ = 9.86 f 0.20 S;vzo = 0.752 f 0.011 cc per g; and molecular weight = 244,000 f 9,000. Calculations based on these parameters give a frictional ratio, f/f0 = 1.27 f 0.11, and a Stokes radius of 53.0 f 4.8 A. The sedimentation con...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62188-x